Journal: International Journal of Molecular Sciences

Article Title: Discovery of Anti-Coronavirus Cinnamoyl Triterpenoids Isolated from Hippophae rhamnoides during a Screening of Halophytes from the North Sea and Channel Coasts in Northern France

doi: 10.3390/ijms242316617

Figure Lengend Snippet: Cytotoxicity of the methanolic crude extracts on Huh-7 cells. Huh-7 cells were treated with two concentrations, 25 and 100 μg/mL, of crude extracts. Control cells were treated with 0.1% DMSO only. The cells were incubated for 24 h, and an MTS assay was then performed to determine the cell viability. The data bars represent the mean ± standard error of the mean (SEM) of three experiments performed in triplicate. The asterisk indicates a statistical difference compared to the control. (**, p < 0.01; ***, p < 0.001). (L) = Leaves, (S) = Stem, (R) = Roots.

Article Snippet: Huh-7 cells and Vero-81 cells were seeded in 96-well plates and incubated with 100 μL of culture medium containing increasing concentrations of our compounds for 24 h. An MTS based viability assay (CellTiter 96 aqueous nonradioactive cell proliferation assay, Promega, Madison WI, USA) was performed, as recommended by the manufacturer.

Techniques: Control, Incubation, MTS Assay

Journal: Advanced healthcare materials

Article Title: Nano-engineered particles for enhanced intra-articular retention and delivery of proteins

doi: 10.1002/adhm.201400051

Figure Lengend Snippet: A) Dynamic light scattering sizing of nanoparticles, measured using the refractive index for water (1.33). B) Scanning electron microscopy (SEM) images of nanoparticles. Scale: 200 nm. C) Particle stability in 1% serum conditions. D) Metabolic activity assay for cytotoxicity for cells incubated with increasing dose of nanoparticles. E) SEM images of Fibronectin nanoparticles (FN-NP, Scale: 200 nm) and the functional ability of resulting FN-NPs in binding to HFN antibody. ANOVA p<0.0001; Pairwise: FN-NP + HFN vs. FN-NP: p<0.001; FN-NP + HFN vs. FN-NP: p<0.001; FN-NP + HFN vs. FN-NP + IgG: p<0.01; FN-NP + HFN vs. BSA-NP + HFN: p<0.001.

Article Snippet: In vitro cytotoxicity assay was performed using a cell metabolic activity assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega).

Techniques: Refractive Index, Electron Microscopy, Metabolic Assay, Incubation, Functional Assay, Binding Assay